Laboratory of Bioluminescent Biotechnologies
Department of Biophysics
School of Fundamental Biology and Biotechnology
Siberian Federal University
CORE RESEARCH AREAS
Microbiology, Enzymology, Environmental Biotechnology, Biosensor, Molecular Biology
WORK DONE IN BRIEF Ph.D. thesis on the topic entitled “STUDIES ON LUCIFERASE ENZYMES FOR THEIR APPLICATION IN ASSESSING HYGIENE STATE OF SELECTED FOOD SAMPLES”
Luciferase enzyme from indigenously available fireflies (Asymmetricata humeralis walker) was extracted which has been not characterized till date. Purification of firefly luciferase enzyme from these luminescent insect was carried out which was utilized for hygiene monitoring, rapid determination milk and fish freshness. Further, an In-house avalanche photodiode and photomultiplier tube based bioluminometer was fabricated for the development of optical biosensor.
AUGUST 2009 – AUGUST 2011
Position: Junior Research Fellow at CSIR-CFTRI, Mysore, India.
WORK DONE IN BRIEF FOR Ph.D. thesis on the topic entitled “STUDIES ON LUCIFERASE ENZYMES FOR THEIR APPLICATION IN ASSESSING HYGIENE STATE OF SELECTED FOOD SAMPLES”
Rapid prescreening of hazardous materials (Heavy metals and pesticides) present in food and water samples was carried out using whole cell bioluminescent bacteria having in vivo luciferase activity (P. leiognathi) after immobilization. For this, potent bioluminescent strain was isolated from marine mussels and were characterized biochemically and through 16S rRNA sequencing. Parametric optimization of growth and nutrient conditions were carried out for enhanced luminescence emission from P. leiognathi. Further, luciferase enzyme from P. leiognathi was extracted and biochemical parameters were optimized
Rajeev Ranjan, Navin K. Rastogi, M.S. Thakur (2012) Development of immobilized biophotonic beads consisting of Photobacterium leiognathi for the detection of heavy metals and pesticide, Journal of Hazardous Materials 225–226:114– 123 (IF. 4.331)
Rajeev Ranjan, B.S. Priyanka, M.S. Thakur (2014) ATPase inhibitor based luciferase assay for prolonged and enhanced ATP pool measurement as an efficient fish freshness indicator, Analytical and Bioanalytical Chemistry 406:4541–4549 (IF. 3.578)
Abhijith K.S., Richa Sharma, Rajeev Ranjan, M.S. Thakur (2014) Facile synthesis of gold-silver alloy nanoparticles for application in metal enhanced bioluminescence, Photochemical and Photobiological Sciences 13:986-991 (IF. 2.939)
Thakur, M.S.; Ranjan, Rajeev; Aaydha, Vinayaka; Kunhitlu, Abhijith; Sharma, Richa (2013) Nanoparticles and Biophotonics as efficient tools in resonance energy transfer based biosensing for monitoring food toxins and pesticides In: Park B., Appell, M.: Advances in Applied Nanotechnology for Agriculture Vol.1143, Chapter 4, pp 55-85, American Chemical Society.
Thakur, M.S., Vinayaka A.C., Sagaya Selva Kumar, Abhijith K., Rajeev Ranjan, Akshath U.S. (2011) Monograph on biosensors, (ed.): Muralikrishna Reddy, L.V., National Design Research Forum, The Institution of Engineers, Bangalore, pp 1-56.
GENBANK SUBMISSION (NCBI)
Ranjan, R. and Thakur, M.S., GenBank: KC617878.1: Photobacterium leiognathi strain CFTRI-BIOPHOTO-1 16S ribosomal RNA gene, partial Sequence
POSTERS/PAPER PRESENTED AT NATIONAL/INTERNATIONAL CONFERENCE
R. Ranjan, B.S. Priyanka, M.S. Thakur, ATPase Inhibitor based Firefly Luciferase Biosensing Assay Format as an Efficient Fish Freshness Indicator, 3rd International Bio-Sensing Technology Conference, May 12-15, 2013, Sitges, Spain
Savita Kumari R., Preethi. C, Rajeev Ranjan, Prakash Halami, M.S. Thakur, Novel Biosensing Approach for Rapid Detection of Antibiotic Resistant Bacteria using Biophotonics, 7th International Food Convention (IFCON), December 18-21, 2013, Venue: CSIR-CFTRI Campus, Mysore, Karnataka, India
Richa Sharma, Rajeev Ranjan, Abhijith K.S., M.S. Thakur (2012), Principle of energy transfer for enhancement of bioluminescence by gold nanoparticles, In 5th Bangalore Nano, December 5-7, Bangalore
Abhijith K.S., Richa Sharma, Rajeev Ranjan, Plasmon enhanced bioluminescence phenomenon with gold-silver alloy nanoparticles for monitoring toxicants in food 7th International Food Convention (IFCON), December 18-21, 2013, Venue: CSIR-CFTRI Campus, Mysore, Karnataka, India
Rajeev Ranjan, Sanjana Tomar, M.S. Thakur (2012), Can ATP Bioluminescence Technology Recommend a Sanitizing Formulation? International Conference on Industrial Biotechnology & IX Convention of The Biotech Research Society, India & Indo-Italian Workshop on Food Biotechnology: Industrial Processing, Safety & Health, November 21-23.
Rajeev Ranjan, Neeraj Katiyar, M.S. Thakur (2011), Bacteria as Biophotonic Sensor: A Promising Tool for Monitoring Environmental Pollutants, AMI-2011 conference, November 3–6, 2011 in Panjab University, Chandigarh.
Rajeev Ranjan and M.S. Thakur (2011) Biophotonics based biosensor as an efficient tool for monitoring food hygiene and sanitation Indo Swiss Collaboration in Biotechnology, ISCB (March 10-11).
Rajeev Ranjan, M.S. Thakur, L. Sagaya Selva Kumar (2010), Biophotonics and their applications in food and environmental monitoring, 16th International Symposium on Bioluminescence and chemiluminescence, ISBC-2010, April 19-23.
Priyanka B.S., Rajeev Ranjan, M.S. Thakur (2012) Rapid detection of viable bacterial load in milk using ATP bioluminescence, XXII Indian Convention of Food Scientists & Technologists (ICFoST), December 6-7, 2012, Venue: CSIR-CFTRI Campus, Mysore, Karnataka, India
Rajeev Ranjan, M.S. Thakur, Jaivik Prakash (Biophotonics): A simple tool for detection of hazardous materials and sanitary condition at rural level, Gandhian Young Technological Innovation Award/Appreciation 2014, March 29, IIM-A, Ahmedabad.
Entitled studentship from the Department of Biotechnology (DBT) while pursuing M.Sc. course (2007-2009) after qualifying Combined Entrance Examination for Biotechnology (CEEB), Jawaharlal Nehru University, New Delhi, INDIA
Awarded University Grants Commission-Junior Research fellowship after qualifying National Eligibility Test (NET) of (CSIR-UGC), 2008
Awarded travel grant from DBT for presenting a part of my doctoral research work entitled "ATPase Inhibitor based Firefly Luciferase Biosensing Assay Format as an Efficient Fish Freshness Indicator", 3rd International Bio-Sensing Technology Conference, May 12-15, 2013, Sitges, Spain
Upgraded to avail UGC-Senior Research fellowship (2011-2014)
Recipient of Prestigious Gandhian Young Technological Innovation Award- 2014 felicitated by an eminent Indian scientist and Padma Vibhushana, Dr. R.A. Mashelkar, FRS at Indian Institute of Management, Ahmedabad, India.
Shortlisted among top 16 young innovator in DuPont and ET Now Times sponsored power of Shunya contest after peer reviewing and interviewing process for my work on biosensor. The media coverage was telecasted worldwide on ET Now Times Group.
Won three year (2016-2018) grant (RFFI: No.16-34-60100) entitled "Functional nanomaterials for environmental and biomedical applications" from the Russian foundation for Basic research (RFBR), Russian Federation.